Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.
Identifieur interne : 000A70 ( Main/Exploration ); précédent : 000A69; suivant : 000A71Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.
Auteurs : Allison C. Galassie [États-Unis] ; Johannes B. Goll [États-Unis] ; Parimal Samir [États-Unis] ; Travis L. Jensen [États-Unis] ; Kristen L. Hoek [États-Unis] ; Leigh M. Howard [États-Unis] ; Tara M. Allos [États-Unis] ; Xinnan Niu [États-Unis] ; Laura E. Gordy [États-Unis] ; C Buddy Creech [États-Unis] ; Heather Hill [États-Unis] ; Sebastian Joyce [États-Unis] ; Kathryn M. Edwards [États-Unis] ; Andrew J. Link [États-Unis]Source :
- Proteomics [ 1615-9861 ] ; 2017.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques, Cartes d'interactions protéiques, Cellules cultivées, Cellules tueuses naturelles (cytologie), Cellules tueuses naturelles (immunologie), Cellules tueuses naturelles (métabolisme), Granulocytes neutrophiles (cytologie), Granulocytes neutrophiles (immunologie), Granulocytes neutrophiles (métabolisme), Grippe humaine (), Grippe humaine (immunologie), Humains, Lymphocytes B (cytologie), Lymphocytes B (immunologie), Lymphocytes B (métabolisme), Lymphocytes T (cytologie), Lymphocytes T (immunologie), Lymphocytes T (métabolisme), Monocytes (cytologie), Monocytes (immunologie), Monocytes (métabolisme), Protéome (métabolisme), Protéomique, Présentation d'antigène, Sous-type H5N1 du virus de la grippe A (immunologie), Vaccins antigrippaux (usage thérapeutique).
- MESH :
- cytologie : Cellules tueuses naturelles, Granulocytes neutrophiles, Lymphocytes B, Lymphocytes T, Monocytes.
- immunologie : Cellules tueuses naturelles, Granulocytes neutrophiles, Grippe humaine, Lymphocytes B, Lymphocytes T, Monocytes, Sous-type H5N1 du virus de la grippe A.
- métabolisme : Cellules tueuses naturelles, Granulocytes neutrophiles, Lymphocytes B, Lymphocytes T, Monocytes, Protéome.
- usage thérapeutique : Vaccins antigrippaux.
- Adjuvants immunologiques, Cartes d'interactions protéiques, Cellules cultivées, Grippe humaine, Humains, Protéomique, Présentation d'antigène.
English descriptors
- KwdEn :
- Adjuvants, Immunologic, Antigen Presentation, B-Lymphocytes (cytology), B-Lymphocytes (immunology), B-Lymphocytes (metabolism), Cells, Cultured, Humans, Influenza A Virus, H5N1 Subtype (immunology), Influenza Vaccines (therapeutic use), Influenza, Human (immunology), Influenza, Human (prevention & control), Killer Cells, Natural (cytology), Killer Cells, Natural (immunology), Killer Cells, Natural (metabolism), Monocytes (cytology), Monocytes (immunology), Monocytes (metabolism), Neutrophils (cytology), Neutrophils (immunology), Neutrophils (metabolism), Protein Interaction Maps, Proteome (metabolism), Proteomics, T-Lymphocytes (cytology), T-Lymphocytes (immunology), T-Lymphocytes (metabolism).
- MESH :
- chemical , metabolism : Proteome.
- chemical , therapeutic use : Influenza Vaccines.
- chemical : Adjuvants, Immunologic.
- cytology : B-Lymphocytes, Killer Cells, Natural, Monocytes, Neutrophils, T-Lymphocytes.
- immunology : B-Lymphocytes, Influenza A Virus, H5N1 Subtype, Influenza, Human, Killer Cells, Natural, Monocytes, Neutrophils, T-Lymphocytes.
- metabolism : B-Lymphocytes, Killer Cells, Natural, Monocytes, Neutrophils, T-Lymphocytes.
- prevention & control : Influenza, Human.
- Antigen Presentation, Cells, Cultured, Humans, Protein Interaction Maps, Proteomics.
Abstract
Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.
DOI: 10.1002/pmic.201600453
PubMed: 28508465
Affiliations:
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Galassie</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, Vanderbilt University, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, Vanderbilt University, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Goll, Johannes B" sort="Goll, Johannes B" uniqKey="Goll J" first="Johannes B" last="Goll">Johannes B. Goll</name><affiliation wicri:level="2"><nlm:affiliation>The Emmes Corporation, Rockville, MD, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Emmes Corporation, Rockville, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Samir, Parimal" sort="Samir, Parimal" uniqKey="Samir P" first="Parimal" last="Samir">Parimal Samir</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Jensen, Travis L" sort="Jensen, Travis L" uniqKey="Jensen T" first="Travis L" last="Jensen">Travis L. Jensen</name><affiliation wicri:level="2"><nlm:affiliation>The Emmes Corporation, Rockville, MD, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Emmes Corporation, Rockville, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Hoek, Kristen L" sort="Hoek, Kristen L" uniqKey="Hoek K" first="Kristen L" last="Hoek">Kristen L. Hoek</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Howard, Leigh M" sort="Howard, Leigh M" uniqKey="Howard L" first="Leigh M" last="Howard">Leigh M. Howard</name><affiliation wicri:level="2"><nlm:affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Allos, Tara M" sort="Allos, Tara M" uniqKey="Allos T" first="Tara M" last="Allos">Tara M. Allos</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Niu, Xinnan" sort="Niu, Xinnan" uniqKey="Niu X" first="Xinnan" last="Niu">Xinnan Niu</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Gordy, Laura E" sort="Gordy, Laura E" uniqKey="Gordy L" first="Laura E" last="Gordy">Laura E. Gordy</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Creech, C Buddy" sort="Creech, C Buddy" uniqKey="Creech C" first="C Buddy" last="Creech">C Buddy Creech</name><affiliation wicri:level="2"><nlm:affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Hill, Heather" sort="Hill, Heather" uniqKey="Hill H" first="Heather" last="Hill">Heather Hill</name><affiliation wicri:level="2"><nlm:affiliation>The Emmes Corporation, Rockville, MD, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Emmes Corporation, Rockville, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Joyce, Sebastian" sort="Joyce, Sebastian" uniqKey="Joyce S" first="Sebastian" last="Joyce">Sebastian Joyce</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Edwards, Kathryn M" sort="Edwards, Kathryn M" uniqKey="Edwards K" first="Kathryn M" last="Edwards">Kathryn M. Edwards</name><affiliation wicri:level="2"><nlm:affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Link, Andrew J" sort="Link, Andrew J" uniqKey="Link A" first="Andrew J" last="Link">Andrew J. Link</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, Vanderbilt University, Nashville, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, Vanderbilt University, Nashville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Antigen Presentation</term><term>B-Lymphocytes (cytology)</term><term>B-Lymphocytes (immunology)</term><term>B-Lymphocytes (metabolism)</term><term>Cells, Cultured</term><term>Humans</term><term>Influenza A Virus, H5N1 Subtype (immunology)</term><term>Influenza Vaccines (therapeutic use)</term><term>Influenza, Human (immunology)</term><term>Influenza, Human (prevention & control)</term><term>Killer Cells, Natural (cytology)</term><term>Killer Cells, Natural (immunology)</term><term>Killer Cells, Natural (metabolism)</term><term>Monocytes (cytology)</term><term>Monocytes (immunology)</term><term>Monocytes (metabolism)</term><term>Neutrophils (cytology)</term><term>Neutrophils (immunology)</term><term>Neutrophils (metabolism)</term><term>Protein Interaction Maps</term><term>Proteome (metabolism)</term><term>Proteomics</term><term>T-Lymphocytes (cytology)</term><term>T-Lymphocytes (immunology)</term><term>T-Lymphocytes (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Cartes d'interactions protéiques</term><term>Cellules cultivées</term><term>Cellules tueuses naturelles (cytologie)</term><term>Cellules tueuses naturelles (immunologie)</term><term>Cellules tueuses naturelles (métabolisme)</term><term>Granulocytes neutrophiles (cytologie)</term><term>Granulocytes neutrophiles (immunologie)</term><term>Granulocytes neutrophiles (métabolisme)</term><term>Grippe humaine ()</term><term>Grippe humaine (immunologie)</term><term>Humains</term><term>Lymphocytes B (cytologie)</term><term>Lymphocytes B (immunologie)</term><term>Lymphocytes B (métabolisme)</term><term>Lymphocytes T (cytologie)</term><term>Lymphocytes T (immunologie)</term><term>Lymphocytes T (métabolisme)</term><term>Monocytes (cytologie)</term><term>Monocytes (immunologie)</term><term>Monocytes (métabolisme)</term><term>Protéome (métabolisme)</term><term>Protéomique</term><term>Présentation d'antigène</term><term>Sous-type H5N1 du virus de la grippe A (immunologie)</term><term>Vaccins antigrippaux (usage thérapeutique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Adjuvants, Immunologic</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules tueuses naturelles</term><term>Granulocytes neutrophiles</term><term>Lymphocytes B</term><term>Lymphocytes T</term><term>Monocytes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>B-Lymphocytes</term><term>Killer Cells, Natural</term><term>Monocytes</term><term>Neutrophils</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules tueuses naturelles</term><term>Granulocytes neutrophiles</term><term>Grippe humaine</term><term>Lymphocytes B</term><term>Lymphocytes T</term><term>Monocytes</term><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Influenza A Virus, H5N1 Subtype</term><term>Influenza, Human</term><term>Killer Cells, Natural</term><term>Monocytes</term><term>Neutrophils</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>B-Lymphocytes</term><term>Killer Cells, Natural</term><term>Monocytes</term><term>Neutrophils</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules tueuses naturelles</term><term>Granulocytes neutrophiles</term><term>Lymphocytes B</term><term>Lymphocytes T</term><term>Monocytes</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Vaccins antigrippaux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Antigen Presentation</term><term>Cells, Cultured</term><term>Humans</term><term>Protein Interaction Maps</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Cartes d'interactions protéiques</term><term>Cellules cultivées</term><term>Grippe humaine</term><term>Humains</term><term>Protéomique</term><term>Présentation d'antigène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Galassie, Allison C" sort="Galassie, Allison C" uniqKey="Galassie A" first="Allison C" last="Galassie">Allison C. Galassie</name></region><name sortKey="Allos, Tara M" sort="Allos, Tara M" uniqKey="Allos T" first="Tara M" last="Allos">Tara M. Allos</name><name sortKey="Creech, C Buddy" sort="Creech, C Buddy" uniqKey="Creech C" first="C Buddy" last="Creech">C Buddy Creech</name><name sortKey="Edwards, Kathryn M" sort="Edwards, Kathryn M" uniqKey="Edwards K" first="Kathryn M" last="Edwards">Kathryn M. Edwards</name><name sortKey="Goll, Johannes B" sort="Goll, Johannes B" uniqKey="Goll J" first="Johannes B" last="Goll">Johannes B. Goll</name><name sortKey="Gordy, Laura E" sort="Gordy, Laura E" uniqKey="Gordy L" first="Laura E" last="Gordy">Laura E. Gordy</name><name sortKey="Hill, Heather" sort="Hill, Heather" uniqKey="Hill H" first="Heather" last="Hill">Heather Hill</name><name sortKey="Hoek, Kristen L" sort="Hoek, Kristen L" uniqKey="Hoek K" first="Kristen L" last="Hoek">Kristen L. Hoek</name><name sortKey="Howard, Leigh M" sort="Howard, Leigh M" uniqKey="Howard L" first="Leigh M" last="Howard">Leigh M. Howard</name><name sortKey="Jensen, Travis L" sort="Jensen, Travis L" uniqKey="Jensen T" first="Travis L" last="Jensen">Travis L. Jensen</name><name sortKey="Joyce, Sebastian" sort="Joyce, Sebastian" uniqKey="Joyce S" first="Sebastian" last="Joyce">Sebastian Joyce</name><name sortKey="Link, Andrew J" sort="Link, Andrew J" uniqKey="Link A" first="Andrew J" last="Link">Andrew J. Link</name><name sortKey="Niu, Xinnan" sort="Niu, Xinnan" uniqKey="Niu X" first="Xinnan" last="Niu">Xinnan Niu</name><name sortKey="Samir, Parimal" sort="Samir, Parimal" uniqKey="Samir P" first="Parimal" last="Samir">Parimal Samir</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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